BIOLOGY (Code No. 044)
COURSE STRUCTURE
CLASS XII (2021-22) (PRACTICAL)
A. List of Experiments
1. Prepare a temporary mount to observe pollen germination.
2. Collect and study soil from at least two different sites and study them for texture, moisture content, pH and water holding capacity. Correlate with the kinds of plants found in them.
3. Collect water from two different water bodies around you and study them for pH, clarity and presence of any living organism.
4. Study the presence of suspended particulate matter in air at two widely different sites.
5. Study the plant population density by quadrat method.
6. Study the plant population frequency by quadrat method.
7. Prepare a temporary mount of onion root tip to study mitosis.
8. Study the effect of different temperatures and three different pH on the activity of salivary amylase on starch.
9. Isolate DNA from available plant material such as spinach, green pea seeds, papaya, etc.
B. Careful observation of the following (Spotting):
1. Flowers adapted to pollination by different agencies (wind, insects, birds).
2. Pollen germination on stigma through a permanent slide or scanning electron micrograph.
3. Identification of stages of gamete development, i.e., T.S. of testis and T.S. of ovary through permanent slides (from grasshopper/mice).
4. Meiosis in onion bud cell or grasshopper testis through permanent slides.
5. T.S. of blastula through permanent slides (Mammalian).
6. Mendelian inheritance using seeds of different colour/sizes of any plant.
7. Prepared pedigree charts of any one of the genetic traits such as rolling of tongue, blood groups,ear lobes, widow's peak and colourblindness.
8. Controlled pollination - emasculation, tagging and bagging.
9. Common disease causing organisms like Ascaris, Entamoeba, Plasmodium, any fungus causing ringworm through permanent slides, models or virtual images. Comment on symptoms of diseases that they cause.
10. Two plants and two animals (models/virtual images) found in xeric conditions. Comment upon their morphological adaptations.
11. Two plants and two animals (models/virtual images) found in aquatic conditions. Comment upon their morphological adaptations.
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